Protein Characterization
Thread Rating:
  • 0 Vote(s) - 0 Average
  • 1
  • 2
  • 3
  • 4
  • 5
seminar class
Active In SP

Posts: 5,361
Joined: Feb 2011
26-02-2011, 12:03 PM

V. Praveen Kumar

.ppt   protein characterization.ppt (Size: 685 KB / Downloads: 89)
Protein Characterization
Why do we analyse proteins
• Proteins play crucial roles in nearly all biological processes. These many functions of proteins are a result of the folding of proteins into many distinct 3D structures.
• Protein analysis tries to explore how amino acid sequences specify the structure of proteins and how these proteins bind to substrates and other molecules to perform their functions.
• Protein analysis allows us to understand the function of the protein based on its structure
Main Steps in Protein Analysisà
1. Extraction of proteins.
2. Purification of the protein.
3. Structural Characterization of the protein.
1. Protein Extraction
2. Involves the efficient extraction of the protein and peptide in a biologically active form from tissue.
3. During this process, inactivation of proteolytic enzymes is required. Why ? Not to cause degradation of the proteins contained in the tissue.
4. Extraction of protein: homogenize it in a physiological buffer (0.05M NaPO4, PH 7.4) containing proteolytic enzyme inhibitors (EDTA, Pepstatin)
5. Extraction of peptides: boil the tissue with 1M Acetic Acid for 5 min à homogenize tissue in ethanol / 0.1MHCl (ratio 3:1) at 0°C. This will burst open the vesicles to release the peptides in solution.
2. Protein Purification
Proteins can be purified according to certain properties they possess. These properties allow us to employ different techniques in purifying proteins.
A. Differential Precipitation
• Precipitation: the process of formation of a solid that was previously held in solution. The solid is separated from the solution.
• When NH4 SO4 or polyethylene glycol are added to a protein solution, a precipitate forms and it can be separated from the solution after centrifugation.
• If the concentrations of NH4 SO4 or polyethylene glycol are increased, more precipitate forms.
B. Gel Filtration Chromatography
• Also called Gel Permeation Chromatography.
• Separates protein molecules according to their molecular size.
• The solution is inserted to the top of a specialized column.
• This column consists of specialized porous beads.
• Small molecules of protein enter the beads while large molecules can’t and stay in the space between the beads.
• Therefore, large molecules flow more rapidly through the column and emerge first from the bottom of the column.
• Advantage: larger quantities of proteins can be separated.
• Disadvantage: Lower resolution.
C. Ion Exchange Chromatography
• Separates protein molecules according to their molecular charge.
• In this technique, the beads of the column have a specific charge on them. This is a result of a molecule that is attached to these beads.
• The beads might be +ve charged by attaching them to DEAE (diethylaminoethyl) cellulose or –ve charged by attaching them to carboxymethyl cellulose.
• The beads in the column depend on the protein that you want to purify.
• If the protein is –ve charged then you have to use positively charged beads and vice versa.
How does it work?
• For example, if the protein of interest is negatively charged, then you will use a DEAE-cellulose column.
• The protein will bind to the positively charged beads.
• This protein that is attached to the beads can be released by increasing the concentration of NaCl (or other salt).
• The Na+ ions (or other cation) will compete and bind to the beads in the column instead of the protein.
• Proteins that are highly positively charged will emerge first because they will be repelled by the beads.
D. Reversed Phase HPLC
• This technique purifies proteins according to their hydrophobicity.
• HPLC – High Pressure Liquid Chromatography
• Reverse phase chromatography is a form of chromatography in which the stationary phase is hydrophobic and the mobile phase is more hydrophilic than the stationary phase. This is "reversed" from normal phase chromatography, in which the opposite is the case.
• The protein solution is pumped into a column containing Silica beads with attached hydrocarbon chain groups.
• These hydrocarbon groups may differ. Example: Octacecyl, Butyl, Propyl, Phenyldimethyl.
• The hydrophobic groups of the protein will bind to the beads.
• The hydrophilic proteins will emerge from the column first.
• There are many different sizes of columns with varying widths:
Preperative (2.5-5cm) â Analytical (0.4-1cm) â Microbore (0.1-0.2cm)
• The solvents used:
– Aqueous Phase: Water + 0.1% Triflouroacetic acid
– Organic Phase: Acetonitrile
E. Affinity Chromatography
• It is the most powerful means of purifying proteins.
• It takes the advantage of the high affinity of many proteins to specific chemical groups or molecules.
• Example:
Concavalin A is a glucose binding protein. It can be purified by passing it through a column that has special beads. These beads have glucose attached to them.
The glucose binding protein binds to the beads and other proteins don’t.
The protein can be released from the beads by increasing the concentration of glucose. This will displace the glucose binding protein from the beads.


Important Note..!

If you are not satisfied with above reply ,..Please


So that we will collect data for you and will made reply to the request....OR try below "QUICK REPLY" box to add a reply to this page

Quick Reply
Type your reply to this message here.

Image Verification
Please enter the text contained within the image into the text box below it. This process is used to prevent automated spam bots.
Image Verification
(case insensitive)

Possibly Related Threads...
Thread Author Replies Views Last Post
  complexation and protein binding jaseelati 0 364 17-02-2015, 03:18 PM
Last Post: jaseelati
  Expression of efflux pump protein MSMEG_1427 of Mycobacterium smegmatis study tips 0 650 21-08-2013, 12:59 PM
Last Post: study tips
  CHARACTERIZATION OF Triticum Dicoccoides GERMPLASM FOR HIGH GRAIN PROTEIN GENE REPORT study tips 0 741 25-06-2013, 12:49 PM
Last Post: study tips
  Nonlinear Elasto-Mammography for Characterization of Breast Tissue Properties pdf study tips 0 742 05-03-2013, 10:20 AM
Last Post: study tips
  Denoising, Segmentation and Characterization of Brain Tumor from Digital MR Images project girl 0 670 06-11-2012, 05:57 PM
Last Post: project girl