(Sodium dodecyl Sulphate Polyacrylamide Gel Electrophoresis)
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15-10-2010, 11:31 AM

.ppt   Dr. A.G.IngalePresentation on SDS PAGE.ppt (Size: 1.68 MB / Downloads: 68)
Presented By
Associate professor
Department Of Biotechnology

(Sodium dodecyl Sulphate Polyacrylamide Gel Electrophoresis)

What is SDS-PAGE?

SDS (Sodium dodecyl Sulphate) is a detergent used to denature proteins and give them a negative charge
PAGE: Polyacrylamide Gel Electrophoresis

It is a technique to separate proteins by their molecular weight

What is in the Sample Buffer?
►Tris buffer to provide appropriate pH
►SDS (sodium dodecyl sulphate) detergent to dissolve proteins and give them a negative charge
►Glycerol to make samples sink into wells
►Bromophenol Blue dye to visualize samples
Why Use Acrylamide Gels to Separate Proteins?

Acrylamide gel: tight matrix
Ideal for protein separation
Smaller pore size than agarose
Proteins much smaller than intact
chromosonal DNA
average amino acid =
110 Dalton

Why do we need to denature the proteins?

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