confocal microscopy
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Hira Aslam
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#1
18-01-2011, 06:26 PM


i want material regarding confocal microscopy i-e its principle,basics,how to use, applications etc...send me soon on my id..thanks
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seminar surveyer
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#2
19-01-2011, 11:03 AM



By:
Denis Semwogerere
Eric R. Weeks
Emory University, Atlanta, Georgia, U.S.A



INTRODUCTION
A confocal microscope creates sharp images of a speci- men that would otherwise appear blurred when viewed with a conventional microscope. This is achieved by excluding most of the light from the specimen that is not from the microscope’s focal plane. The image has less haze and better contrast than that of a conven- tional microscope and represents a thin cross-section of the specimen. Thus, apart from allowing better observation of fine details it is possible to build three-dimensional (3D) reconstructions of a volume of the specimen by assembling a series of thin slices taken along the vertical axis

MODERN CONFOCAL MICROSCOPY
Modern confocal microscopes have kept the key ele- ments of Minsky’s design: the pinhole apertures and point-by-point illumination of the specimen. Advances in optics and electronics have been incorporated into current designs and provide improvements in speed, image quality, and storage of the generated images. Although there are a number of different confocal microscope designs, this entry will discuss one general type—the other designs are not markedly different. The majority of confocal microscopes image either by reflecting light off the specimen or by stimulating fluorescence from dyes (fluorophores) applied to the specimen. The focus of this entry will be on fluores- cence confocal microscopy as it is the mode that is most commonly used in biological applications. The difference between the two techniques is small. There are methods that involve transmission of light through the specimen, but these are much less common.


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